The regional fare card known as the Connect Card is now accepted on Yolobus, in addition to Sacramento Regional Transit and other transit systems in the region.  Unfortunately, the Connect Card is not an accepted form of payment on Unitrans.  This is because the expensive card reading technology is not being installed on Unitrans buses, as this was not cost-effective given the relatively small number of regional transit riders.  If you use a Connect Card on Yolobus and wish to continue your trip on Unitrans, please request a printed transfer from the Yolobus driver and present that on Unitrans.  If the transfer time and date are valid, it will be accepted on Unitrans and no additional fare is required.  If you are transferring from Unitrans to Yolobus, you will need to pay the $1 fare on Unitrans (or use a 10-ride ticket), and then use your Connect Card to board Yolobus.

From Unitrans FAQs:

I have a Yolobus / Sacramento Regional Transit pass. Can I use it on Unitrans?

Unitrans accepts printed Yolobus and Sacramento Regional Transit (Sac RT) passes and transfers. Unitrans also accepts Amtrak and Fairfield/Suisun Transit passes and transfers as valid fare.  However, electronic-only media such as Yolobus stored value cards and Sacramento Connect Card cannot be used on Unitrans as valid fare.  That is because they cannot be verified by the bus driver.  If you are using a Connect Card on Yolobus and wish to transfer to Unitrans, please request a printed transfer from the Yolobus driver, and then use that as your fare when boarding Unitrans.